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python package for registering multimodal whole slide microscopy images

Project description

wsireg

Documentation Status

Multi-modal or mono-modal whole slide image registration in a graph structure for complex registration tasks using elastix.

Features

  • Graph based approach to defining modalities and arbitrary transformation paths between associated images

  • Use of elastix (through ITKElastix) to perform registration

  • Support for linear and non-linear transformation models

  • Transform associated data (masks, shape data) along the same path as the images.

  • Supports images converted to OME-TIFF using bioformats2raw -> raw2ometiff pipeline

  • All registered images exported as pyramidal OME-TIFF or OME-zarr that can be viewed in software such as Vitessce , vizarr, QuPath, OMERO or any platform that supports these formats.

  • All transforms for complex registration paths are internally composited and only 1 interpolation step is performed, avoiding accumulation of interpolation error from many registrations

  • Shape data (polygons, point sets, etc.) in GeoJSON format (future portable format for QuPath detection/annotation data) can be imported and transformations applied producing a modified GeoJSON

  • Some support for reading native WSI formats: currently reads .czi and .scn but could be expanded to other formats supported by python package tifffile

History

0.3.9 (2023-01-10)

Deprecates unused aicsimageio dependency.

0.3.8 (2023-01-10)

Fix bug that occurs when file paths contain a period.

0.3.7 (2022-04-28)

Add support for translation transformation. Fix writing of merge modalities in cmd line configuration.

0.3.6 (2022-04-28)

Fixes bug in wsireg2d console script.

0.3.5 (2022-04-28)

Add support for python 3.10 with updated itk-elastix.

0.3.4 (2022-04-27)

Major refactor of RegImage class, various small improvements throughout to support napari-wsireg.

0.3.2.2 (2022-02-04)

This minor update adds the ability to draw binary or label masks using RegShapes.

0.3.2.1 (2021-12-28)

This releases fixes some small bugs around shape transforms.

  • fix geojson import/export

  • fix bug with geojson reading

0.3.0 (2021-09-22)

  • add “ome.tiff-bytile” writer to write transformed images tile-by-tile

  • unify data reading from tiffs to use dask

  • numerous improvements, bug fixes, and additional tests

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