pytest ngs fixtures
Project description
pytest-ngsfixtures
About
This is a pytest plugin that provides functionality for next generation sequencing pytest fixtures. There are some predefined fixtures, but the main functionality depends on configuring fixtures via the pytest.mark helper function.
See the pytest-ngsfixtures documentation for more information and usage.
Free software: GNU General Public License v3
Features
a small test ngs data set
predefined sample layouts
wrappers for quickly setting up workflow tests
Installation
$ conda install -c percyfal pytest-ngsfixtures
$ pip install pytest-ngsfixturesUsage
You can easily setup a test requiring the predefined samples and ref fixtures:
def test_data(samples, ref):
shell("bwa index {}".format(ref.join("scaffolds.fa")))
shell("bwa mem {} {} {} | samtools view -b > {}".format(
ref.join("scaffolds.fa"),
samples.join("s1_1.fastq.gz"),
samples.join("s1_2.fastq.gz"),
samples.join("s1.bam")
))
assert samples.join("s1.bam").exists()The samples and ref fixtures can also be configured to use local files:
import pytest
@pytest.mark.samples(layout={'s1_1.fastq.gz': "/path/to/read1.fastq.gz",
's1_2.fastq.gz': "/path/to/read2.fastq.gz"})
@pytest.mark.ref(data={'ref.fa': "/path/to/reference.fa"})
def test_data(samples, ref):
# Do something with dataIn addition, there are wrapper functions and fixtures for workflow managers, including Snakemake.
import pytest
from pytest_ngsfixtures.wm.snakemake import snakefile, run as snakemake_run
# By default, the snakefile fixture assumes there is a Snakefile in
# the test file directory
def test_workflow(samples, snakefile):
snakemake_run(snakefile, options=["-d", str(samples)])
assert samples.join("results.txt").exists()See the pytest-ngsfixtures documentation for more examples.
Credits
This package was created with Cookiecutter and the audreyr/cookiecutter-pypackage project template.
History
0.8.1 (2019-09-06)
Hotfix: fix failing conda builds
0.8.0 (2019-09-05)
Features
Add support for python 3.7
Pin pytest >= 5.0
PEP-8 formatting
Check for correct snakemake repo tag
0.7.6 (2018-05-25)
Features
Add path option to override tmpdir_factory invocation
Pass ignore_errors to copy/link function
0.7.5 (2018-05-25)
Features
Add Fixture class to setup fixtures (#63)
0.7.4 (2018-05-24)
Bugfixes
Make pytest.mark.fixture have precedence over pytest.mark.parametrize when updating functions
0.7.3 (2018-05-24)
Features
Add testdir parameter and allow configuration of all fixture options via parametrization (#62)
0.7.2 (2018-05-24)
Bugfixes
Fix layout for multi-run samples (#61)
0.7.1 (2018-05-23)
Bugfixes
Update busybox images for tests (#59)
Fix failing test (#58)
0.7.0 (2018-05-23)
Breaking changes
This is a major update in which the API has changed considerably. Notably, most of the code for generating fixtures has been removed and refactored completely. Most importantly, the factory functions have been replaced by a small number of fixtures that can be configured via the pytest.mark helper.
In addition, most data files have been removed in an attempt to make the package as small as possible. As the location of test data has been decoupled from package functionality, it makes more sense to distribute package data separately.
See the API documentation for more information.
Features
Remove application data (#30)
Simplify config.runfmt_alias function (#56)
Only package tiny sequence data (#55)
Simplify sample layout configuration (#57)
0.6.4 (2018-01-31)
Add docker-py as a dependency (#52)
0.6.3 (2018-01-02)
Remove obsolete working directory argument - requires snakemake >= 4.4.0
Install correct docker API (#50)
Allow modifying path in shell wrapper (#51)
0.6.2 (2017-12-19)
Add shell wrappers and wrappers for easily setting up workflow tests.
Add tutorial.
Bugfixes.
Update pypi badge
Remove tox.ini
Defer setting alias to FixtureFile (#42)
ReadFixtureFile.SM now returns id (#39)
Options ngs_layout and ngs_runfmt are now lists (#41)
Update project layout configuration (#40)
Add function to return runfmt and alias as tuple (#43)
Read 1 and 2 have same id when alias required (#45)
Census is not increased for read 2 (#44)
Add option to only setup sampleinfo (#47)
Add tutorial (#46)
Add working snakemake tests (#8)
0.6.1 (2017-11-22)
Added some fixes that turned out to be necessary for optional performance.
Add fixture that parametrizes over input parameters (#37)
Fix bug that returned wrong number of snakemake targets in application output generation (#36)
Add travis builds (#35)
Fix reference layout error for python 3.5 (#34)
Update option defaults (#32)
Expose fewer predefined fixtures via plugin (#31)
Unify parameter names (#33)
0.6.0 (2017-11-21)
This is a major revision of the code. Several new abstraction classes have been introduced to ease interaction with local test files, along with new factory functions. For backwards compatibility, most factory functions should work as previously. Documentation has been much improved.
Clarify fixture function naming convention (#29)
Expose bulk of factory inner functions (#24)
Create separate module for os-related functions (#27)
Use separate conda build statements to build for different python versions (#23)
0.5.2 (2017-11-16)
Add pool fixtures (#22)
Add safe_copy function (#21)
0.5.1 (2017-10-25)
Change name to pytest-ngsfixtures (#20)
Add bcftools versions 1.4, 1.4.1, 1.5, 1.6
Add bowtie 1.2.1.1
0.5.0 (2017-09-25)
Add star application
Add vsearch application
Add rseqc application
Add snakemake dependency
Update docs
Separate qualimap pe and se output (#12)
Add mapdamage2 (#11)
Fix mapdamage2 missing output (#18)
Use realpath to determine download url (#17)
Rename download_ngsfixtures_data.py to pytest_ngsfixtures_download_data.py
Update versions for samtools
0.4.0 (2017-03-28)
Add picard output data
Add functions for dealing with application fixtures
Move docs to gh-pages
Add pytest_ngsfixtures_add_application.py for templating new applications
Application outputs now implemented as dictionaries for multiple-output applications
0.3.1 (2017-03-03)
Defer download to script, minor bug fixes
Add application_fixtures to config module
Fix sample_alias bug (#4)
Add script download_ngsfixtures_data.py for manual download of data (#5)
0.3.0 (2017-03-02)
Add application data, pool data, and functions for downloading data
Add application data for cutadapt, fastqc, qualimap and samtools
Add pooled sequencing data
Add functionality for downloading large data sets
Improve make targets for easier releases
Minor bug fixes
0.2.0 (2017-02-24)
Add new reference files, test files, and factory functions.
Use snakemake instead of make to generate data files
Add separate test file for factory functions
Add scaffold file with randomly inserted N’s
Improve safe_symlink
Add threads options for running tests
Add Snakefile and rules for generating application files
Add fileset, filetype factory functions
0.1.0 (2017-01-24)
First release on PyPI.
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